In chemistry, spectrophotometry could be the quantifiable study of electromagnetic spectra. It's extra particular than the general expression electromagnetic spectroscopy in that spectrophotometry offers with visible light, near-ultraviolet, and near-infrared. Also, the term doesn't cover time-resolved spectroscopic techniques.

Spectrophotometry entails the use of a spectrophotometer. A spectrophotometer is a photometer (a machine for measuring lgt depth) that will assess intensity being a purpose in the gentle supply wavelength. Essential characteristics of spectrophotometers are spectral bandwidth and linear assortment of assimilation measurement.

Perhaps probably the most typical application of spectrophotometers would be the measurement of light assimilation, but they may be made to assess diffuse or specular reflectance.  

Strictly, even the emission fifty percent of a luminescence instrument is a sort of spectrophotometer.

The use of spectrophotometers spans several scientific fields, for instance physics, chemistry, biochemistry, and molecular biology. They are broadly utilized in several industries which includes printing and forensic examination.


There are two key classes of equipment: single beam and double beam. A dual beam spectrophotometer compares the light intensity in between two gentle paths, one particular path that contains a guide pattern as well as the other the check sample. A solitary beam spectrophotometer measures the relative light depth of your beam prior to and right after a test pattern is inserted. Even though comparison measurements from dual beam instruments are easier and far more steady, single beam devices can possess a bigger dynamic range and are optically simpler and a lot more compact.

Historically, spectrophotometers use a monochromator that contains a diffraction grating to create the analytical array. There are also spectrophotometers that use arrays of photosensors. Specially for infrared spectrophotometers, you can find spectrophotometers that use a Fourier rework method to obtain the spectral facts faster in a very process called Fourier Rework InfraRed.

Spectrophotometer Manufacturers

Below is a short list of companies that manufacture spectrophotometers.  If changes need to be made please contact us.

List is in alphabetical order:

  • Agilent/Hewlett-Packard
  • Beckman Coulter
  • Bio-Rad
  • Eppendorf
  • GE formerly Amersham
  • Hach
  • Hitachi
  • Jasco
  • Ocean Optics
  • Perkin Elmer
  • PG Instruments
  • Shimadzu
  • Thermo Electron
  • Varian