Toluene in Hexane Spectral Bandwidth Reference Standard
Assessing the photometric and wavelength performance of a UV/VIS spectrophotometer is a necessary part of keeping your laboratory in accordance with Good Laboratory Practice (GLP) standards. Understanding and accounting for how expanded uncertainties affect these measurements is a critical component of the calibration process.
Spectral Bandwidth (SBW) refers to the physical slit-width of the spectrophotometer’s monochromator, which has a direct impact on the resolution of the instruments. In turn, spectral resolution is the ability to resolve two neighboring peaks. Reconciling the resolution is an integral component in getting accurate wavelength and photometric readings on your spectrophotometer.
Recognizing this need for periodically assessing SBW and pursuant to European Pharmacopoeia (EP) and United States Pharmacopeia (USP) requirements, Precision Cells has expanded its world-class spectroscopy calibration line with its new Toluene Spectral Bandwidth Reference Standard.
PC’s Toluene in Hexane Spectral Bandwidth Reference Standard is a set containing:
- High-purity solution of 0.02% Toluene in Hexane
- High-purity Hexane blank
The EP recommends comparing the ratio of the peak maxima at 269nm and the minimum at 266nm to determine the spectral resolution of your spectrophotometer.
Determine the resolution by running the toluene in hexane against the hexane blank between 265-270nm and calculate the peak minima and maxima.
Calculate the ratios of the peak to the trough and compare these results with the reference values.
Please note that this is a liquid calibration filter and does not come with the same Lifetime Warranty as PC's Solid State Standards. The PC Toluene in Hexane filter must be recalibrated every year according to NIST guidelines.
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